by Maryam Henein, The Tenpenny Report:
…mosquitoes were anesthetized on ice, and a leg from each mosquito was removed using sharp forceps and immersed into 20??L of sample dilution buffer supplemented with 0.5??L of DNA release reagent. Leg samples in dilution buffer were incubated for 3…
Mosquitoes were forced to salivate for 30?minutes in glass capillaries.
For their complex sci-fi process, they used African green monkey kidney cells along with mouse monoclonal anti-alphavirus antibodies. The DENV-2 genomic fragment was then inserted into a plasmid and transformed by Escherichia coli.