Dr. Andrew Kaufman refutes “isolation” of SARS-Cov-2; he does step-by-step analysis of a typical claim of isolation; there is no proof that the virus exists

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by Jon Rappoport, No More Fake News:

The global medical community has been asserting that “a pandemic is being caused by a virus, SARS-Cov-2.”

But what if the virus doesn’t exist?

People have been asking me for a step-by-step analysis of a mainstream claim of virus-isolation. Well, here it is.

“Isolation” should mean the virus has been separated out from all surrounding material, so researchers can say, “Look, we have it. It exists.”

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I took a typical passage from a published study, a “methods” section, in which researchers describe how they “isolated the virus.” I sent it to Dr. Andrew Kaufman [1], and he provided his analysis in detail.

I found several studies that used very similar language in explaining how “SARS-CoV-2 was isolated.” For example, “Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States, (Emerging Infectious Diseases, Vol. 26, No. 6 — June 2020)” [2].

First, I want to provide a bit of background that will help the reader understand what is going on in the study.

The researchers are creating a soup in the lab. This soup contains a number of compounds. The researchers assume, without evidence, that “the virus” is in this soup. At no time do they separate the purported virus from the surrounding material in the soup. Isolation of the virus is not occurring.

They set about showing that the monkey (and/or human cells) they put in the soup are dying. This cell-death, they claim, is being caused by “the virus.” However, as you’ll see, Dr. Kaufman dismantles this claim.

There is no reason to infer that SARS-CoV-2 is in the soup at all, or that it is killing cells.

Finally, the researchers assert, with no proof or rational explanation, that they were able to discover the genetic sequence of “the virus.”

Here are the study’s statements claiming isolation, alternated with Dr. Kaufman’s analysis:

STUDY: “We used Vero CCL-81 cells for isolation and initial passage…”

KAUFMAN: “Vero cells are foreign cells from the kidneys of monkeys and a source of contamination. Virus particles should be purified directly from clinical samples in order to prove the virus actually exists. Isolation means separation from everything else. So how can you separate/isolate a virus when you add it to something else?”

STUDY: “…We cultured Vero E6, Vero CCL-81, HUH 7.0, 293T, A549, and EFKB3 cells in Dulbecco minimal essential medium (DMEM) supplemented with heat-inactivated fetal bovine serum (5% or 10%)…”

KAUFMAN: “Why use minimal essential media, which provides incomplete nutrition [to the cells]? Fetal bovine serum is a source of foreign genetic material and extracellular vesicles, which are indistinguishable from viruses.”

STUDY: “…We used both NP and OP swab specimens for virus isolation. For isolation, limiting dilution, and passage 1 of the virus, we pipetted 50 μL of serum-free DMEM into columns 2–12 of a 96-well tissue culture plate, then pipetted 100 μL of clinical specimens into column 1 and serially diluted 2-fold across the plate…”

KAUFMAN: “Once again, misuse of the word isolation.”

STUDY: “…We then trypsinized and resuspended Vero cells in DMEM containing 10% fetal bovine serum, 2× penicillin/streptomycin, 2× antibiotics/antimycotics, and 2× amphotericin B at a concentration of 2.5 × 105 cells/mL…”

KAUFMAN: “Trypsin is a pancreatic enzyme that digests proteins. Wouldn’t that cause damage to the cells and particles in the culture which have proteins on their surfaces, including the so called spike protein?”

KAUFMAN: “Why are antibiotics added? Sterile technique is used for the culture. Bacteria may be easily filtered out of the clinical sample by commercially available filters (GIBCO) [3]. Finally, bacteria may be easily seen under the microscope and would be readily identified if they were contaminating the sample. The specific antibiotics used, streptomycin and amphotericin (aka ‘ampho-terrible’), are toxic to the kidneys and we are using kidney cells in this experiment! Also note they are used at ‘2X’ concentration, which appears to be twice the normal amount. These will certainly cause damage to the Vero cells.”

STUDY: “…We added [not isolated] 100 μL of cell suspension directly to the clinical specimen dilutions and mixed gently by pipetting. We then grew the inoculated cultures in a humidified 37°C incubator in an atmosphere of 5% CO2 and observed for cytopathic effects (CPEs) daily. We used standard plaque assays for SARS-CoV-2, which were based on SARS-CoV and Middle East respiratory syndrome coronavirus (MERS-CoV) protocols…”

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